fitc conjugated wga  (Vector Laboratories)

Journal: Frontiers in Immunology

Article Title: Acetylsalicylic acid disrupts SARS-CoV-2 spike protein glycosylation and selectively impairs binding to ACE2

doi: 10.3389/fimmu.2025.1706997

Figure Lengend Snippet: ASA prevents S1-induced lung injury, fibrosis and inflammation in hACE2-KI mice. (A, B) Representative images of lung sections stained with H&E (A) and Masson’s trichrome (B) from mice receiving intratracheal instillation of vehicle, 15 μg S1 pre-treated overnight with vehicle (S1), 15 μg S1 pre-treated overnight with ASA 20 mg/L (ASA-treated S1) or 15 μg S1 pre-treated overnight with vehicle followed by ASA 20 mg/L administered immediately afterward through the same route (S1+ASA) at 7 days (n=3 per group). Scale bars: 100 µm for H&E and 20 µm for Masson’s trichrome staining. (C-E) Representative images and quantification of fibronectin ( C , red), MAC2 + macrophages ( D , red), and GR1 + neutrophils ( E , red) in lung tissue sections of mice receiving intratracheal instillation of vehicle, S1, ASA-treated S1 or S1+ASA at 7 days (n=3 per group). Lung structures and nuclei were counterstained with WGA lectin (green) and DAPI (blue), respectively. Scale bar: 20 µm. Data are expressed as % of fibronectin fluorescence area per high power field at ×63 magnification (% area/field) and the average number of MAC2 + or GR1 + cells per high power field at ×63 magnification. For all panels, results are shown as mean ± SEM and were analyzed with Tukey’s multiple comparison test. *p-value<0.05, **p-value<0.01, and ***p-value<0.001 vs Vehicle; °°p-value<0.01, and °°°p-value<0.001 vs S1; ## p-value<0.01, and ### p-value<0.001 vs ASA-treated S1.

Article Snippet: Lung structure was counterstained with FITC-wheat germ agglutinin (WGA) lectin (Vector Laboratories, FL-1021).

Techniques: Staining, Fluorescence, Comparison, Significance Assay

Journal: Frontiers in Immunology

Article Title: Acetylsalicylic acid disrupts SARS-CoV-2 spike protein glycosylation and selectively impairs binding to ACE2

doi: 10.3389/fimmu.2025.1706997

Figure Lengend Snippet: Glycosylation-deficient S1 double mutant displays reduced lung pathogenicity in vivo . (A, B) Representative images of lung sections stained with H&E (A) and Masson’s trichrome (B) from mice receiving intratracheal instillation of vehicle, 15 μg wild-type S1 (S1 WT), 15 μg single S1 mutant (S1 N61D) or 15 μg double S1 mutant (S1 N61D/S325A) at 7 days (n=3 per group). Scale bars: 100 µm for H&E and 20 µm for Masson’s trichrome staining. (C–E) Representative images and quantification of fibronectin ( C , red), MAC2 + macrophages ( D , red), and GR1 + neutrophils ( E , red) in lung tissue sections of mice receiving intratracheal instillation of vehicle, S1 WT, S1 N61D or S1 N61D/S325A at 7 days (n=3 per group). Lung structures and nuclei were counterstained with WGA lectin (green) and DAPI (blue), respectively. Scale bar: 20 µm. Data are expressed as % of fibronectin fluorescence area per high power field at ×63 magnification (% area/field) and the average number of MAC2 + or GR1 + cells per high power field at ×63 magnification. For all panels, results are shown as mean ± SEM and were analyzed with Tukey’s multiple comparison test. **p-value<0.01, and ***p-value<0.001 vs Vehicle; °p-value<0.05, °°p-value<0.01, and °°°p-value<0.001 vs S1 WT; # p-value<0.05, and ## p-value<0.01 vs S1 N61D.

Article Snippet: Lung structure was counterstained with FITC-wheat germ agglutinin (WGA) lectin (Vector Laboratories, FL-1021).

Techniques: Glycoproteomics, Mutagenesis, In Vivo, Staining, Fluorescence, Comparison, Significance Assay